![]() ![]() Once more, things have changed again and the modern bioprocess in incubator shakers is demanding even more information from the shake flask and better performance from the incubator shaker. LED lighting to encouraged growth of phototrophic cell lines with special spectra.Ģ019: Digitalisation & Performance – The users in the 2020’s want even more When biofuel applications using photosynthetic bacteria and algae to produce fermentable sugars, the Multitron evolved to meet these requirements, yet again. Suddenly, the results of screening could be taken to small scale clinical trials with the output from a single, stacked system. An incubator shaker which could be qualified like a bioreactor for such validated process was a real revolution this time around. Serial communications gave way to networked connectivity to bioprocess software for logging, analysis, and validation of shaker-based processes. Next to the “classical” technical shaker features, influencing culture growth and productivity, usability topics have become more popular from the mid 2010’s onwards. A special high speed, short throw drive system solved this problem. Providing a dequate mixing for oxygen transfer was an issue in the smaller wells. They needed special options and adaptation to accommodate large numbers per chamber and to stop wells drying out over time. The need for thousands of screening experiments to provide rapid screening for specific biological activity drove an increased use in 96-well plates. ![]() ![]() Then, things changed and applications such as algal biofuel and massively parallel screening in microtiter plates came to the fore. The excellent temperature distribution, a certified antimicrobial surface and options such as darkening for insect cells infected with Bacculovirus made the Mulitron the “ideal home” for these cultures.Ģ012-2017: Multiple, parallel experiments and green applications This led to a new generation of Multitron with a larger 50 mm shaking throw for gentle mixing plus the ability to measure and control parameters such as gassing with CO 2 and percentage relative humidity. Also, production of viral vectors for vaccines and recombinant work using both insect and mammalian cells were common. Microbial and fungi/yeast hosts for r ecombinant protein production were limited in their ability to provide complete glycosylation. 2008-2011: Cell culture applications come to the foreīy the early 2000’s, the need for growth of mammalian and insect cells in suspension culture was clear. However, applications were changing with the rise of cell culture for mammalian or insect cell lines. This system was a genuine revolution and met the changing needs of researchers engaged in microbial work. This provided its own design challenges to keep the top deck accessible, providing easy flask handling, prevent any spillages from moving down the stack, and allowing easy access for cleaning and servicing. The first Multitron provided a “game changer” in that it made use of vertical space in the laboratory by stacking up to three incubation chambers. The alternative, an incubator shaker was more flexible, but lacked capacity, both in number of flasks and their maximum size. These systems were simple, effective for their main task but extremely inflexible for accommodating several users with different needs e.g., different growth temperatures. The main application for these units was often large-scale screening for antibiotics. In previous decades, the need for high capacity and throughput was met by free-standing, large shakers housed in constant-temperature rooms. The Multitron development dates to the 1990’s and came from the need for maximum shake flask capacity for the minimum space in the laboratory. ![]() In addition to shake flasks, test tubes and microwell plates were being used with shakers to provide good mixing and oxygen transfer.ġ991: Focus on fungal and microbial applications By the mid 1960’s, the shaker and its temperature-controlled sibling, the incubator shaker, were in common use as a standard laboratory tool for the biosciences. This made the shake flask useful for seed train production when Stirred Tank Reactors (STR’s) first came into use in the 1940’s for antibiotic production. Key parameters like shaking throw, an orbital movement for good growth and speed range were established early. To provide a little context from a historical perspective, the use of orbital shakers for submerged culture in shake flasks dates to the mid 1930’s and concentrated on fungal culture. ![]()
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